Name: Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology, 2701) (en Inglés)
Price: 350520.00 CLP
Availability: InStock
Author: Bhakat Kishor K.,Hazra Tapas K.
ISBN: 9781071633724

portada Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology, 2701) (en Inglés)

Formato

Libro Físico

Editorial

Humana

Autor

Bhakat Kishor K.,Hazra Tapas K.

Idioma

Inglés

N° páginas

275

Encuadernación

Tapa Dura

ISBN13

9781071633724

N° edición

Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology, 2701) (en Inglés)

Bhakat Kishor K.,Hazra Tapas K. (Autor) · Humana · Tapa Dura

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Reseña del libro "Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology, 2701) (en Inglés)"

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.